Platform — Purensis

An integrated system.

Whole-slide imaging, AI-guided segmentation, laser microdissection, and automated fluidic collection combined in a single workflow. Each tissue region is captured with paired imaging, spatial coordinates, and downstream proteomic measurement.

Advanced Imaging System

Inverted Microscope System

Our imaging system provides high-quality tile-based acquisition with intelligent focus optimization:

Multi-tile scanning: Automated panoramic image acquisition across large tissue areas
Z-stack acquisition: Multiple focal planes captured for each tile
Intelligent focus selection: Scharr variance-based algorithm automatically identifies optimal focus plane
Multi-channel support: Brightfield, fluorescence, and other imaging modalities
High-precision stitching: Stitched panoramic images with coordinate metadata

Resolution: Up to 2048×1536 per tile

Pixel Precision: Dynamic calculation based on objective (e.g., 0.645 µm @ 10×)

Scan Area: Full slide coverage (tile-based)

Integrated Image Analysis

Segmentation and AI-Augmented Annotation

Advanced image analysis tools for automated cell and region identification:

Deep learning segmentation: Pre-trained models for various cell types with customizable parameters
Interactive tuning: Real-time preview of segmentation results with adjustable thresholds
Clustering analysis: DBSCAN and K-Means algorithms group similar cells or regions
Polygon modification: Automated dilation, smoothing, and simplification tools
Manual refinement: Draw, edit, and merge annotations with precision measurement tools

Smart Analysis: Our unified analysis interface combines segmentation, clustering, and polygon modification in one streamlined workflow.

Precision Laser Microdissection

Automated Coordinate-Guided Cutting

Our laser system delivers micrometer-scale precision with intelligent automation:

Coordinate transformation: Automatic conversion from pixel annotations to stage coordinates
Laser offset calibration: Alignment workflow ensures cutting accuracy
Variable power control: Separate perimeter cutting and tissue release parameters, configurable per tissue type
Leadscrew positioning: Optimal Z-axis positioning during initialization
Path optimization: Efficient cutting patterns minimize tissue damage

Cutting Speed: 2.0 mm/s (configurable)

Precision: Micrometer-scale accuracy

Power Range: 1-100% (tissue-adaptive)

Integrated Fluidics System

Automated Tissue Transport & Collection

Seamless integration of pumps and liquid handling for high-throughput sample processing:

Pump coordination: Synchronized inlet and outlet pumps for smooth tissue transport
Flow cell integration: Tissue flows through controlled microfluidic environment
Automated liquid handling: Automated collection into 96-well plates with well-specific targeting
Priming workflow: Automated system preparation ensures reliability
Safety features: Waste positioning and depth optimization prevent sample loss

High Throughput: Process and collect hundreds of tissue regions automatically, with each sample precisely tracked and stored.

Complete Automated Workflow

Live View & ROI Selection

Professional live view interface allows users to navigate tissue samples and define regions of interest

Automated Scanning

Multi-tile Z-stack acquisition across entire ROI with multi-channel support

Image Processing

Best focus selection using Scharr variance, followed by precision stitching with coordinate metadata generation

AI Analysis & Annotation

AI segmentation, clustering analysis, and manual annotation refinement in unified interface

Coordinate Export

Annotations automatically transformed from pixel coordinates to precise stage coordinates (µm)

Laser Alignment

Calibration workflow measures and compensates for laser-camera offset

Cut & Retrieve

Automated laser cutting along annotation paths with synchronized tissue transport

Well Collection

Automated liquid handler collects isolated tissues into specific wells for downstream analysis

Platform.